Colistin Susceptibility Testing of Enterobacteriaceae by Agar Dilution (AD), Broth Microdilution (BMD) and Polymyxin NP
نویسندگان
چکیده
2033. Evaluation of the FilmArray Meningitis/Encephalitis Molecular Panel in a Tertiary Care Public County Hospital Tae Hun Kim, MD; Emi Minejima, PharmD; Brad Spellberg, MD; Ira Shulman, MD; Paul Holtom, MD; Tarina Kang, MD and Susan M. Butler-Wu, PhD; Pathology, LAC-USC Medical Center, Los Angeles, California, Pharmacy, USC School of Pharmacy, Los Angeles, California, LAC-USC Medical Center, Los Angeles, California, Clinical Microbiology Laboratory, LAC-USC Medical Center, Los Angeles, California
منابع مشابه
Colistin and Polymyxin B Susceptibility Testing for Carbapenem-Resistant and mcr-Positive Enterobacteriaceae: Comparison of Sensititre, MicroScan, Vitek 2, and Etest with Broth Microdilution.
Colistin and polymyxin B remain part of the last line of antibiotics for multidrug-resistant Gram-negative bacteria, such as carbapenem-resistant Enterobacteriaceae Current joint EUCAST-CLSI recommendations are for broth microdilution (BMD) to be performed for MIC testing of colistin. Commercial susceptibility testing methods were evaluated and compared against the reference BMD, using a suscep...
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In vitro evaluation of colistin susceptibility is fraught with complications, due in part to the inherent cationic properties of colistin. In addition, no reference method has been defined against which to compare the results of colistin susceptibility testing. This study systematically evaluated the available methods for colistin MIC testing in two phases. In phase I, colistin MICs were determ...
متن کاملComparative evaluation of the VITEK 2, disk diffusion, etest, broth microdilution, and agar dilution susceptibility testing methods for colistin in clinical isolates, including heteroresistant Enterobacter cloacae and Acinetobacter baumannii strains.
Increasing antibiotic resistance in gram-negative bacteria has recently renewed interest in colistin as a therapeutic option. The increasing use of colistin necessitates the availability of rapid and reliable methods for colistin susceptibility testing. We compared seven methods of colistin susceptibility testing (disk diffusion, agar dilution on Mueller-Hinton [MH] and Isosensitest agar, Etest...
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OBJECTIVES AND METHODS With their potent activity against Gram-negative bacteria, the polymyxins are important alternative antibiotics for cystic fibrosis (CF) patients. A retrospective evaluation of polymyxin activity against 6001 Pseudomonas aeruginosa, 150 Achromobacter xylosoxidans and 506 Stenotrophomonas maltophilia CF isolates was initiated. In addition, we looked at how polymyxin suscep...
متن کاملInvestigation of whether CLSI broth microdilution method is applicable for MICs Determination of Enterococcus species.
The broth microdilution (BMD) method is an antimicrobial susceptibility testing method defined as a guideline by the Clinical and Laboratory Standards Institute (CLSI). To date, the Japanese Veterinary Antimicrobial Resistance Monitoring System (JVARM), has adopted the agar dilution (AD) method for monitoring antimicrobial resistances targeting isolates of Enterococcus spp., found in the fecal ...
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